Press the Percent T/A selector to select Percent Transmittance or Percent Absorbance mode. Locate the wavelength dial beside the sample chamber and set it to the desired wavelength. Don gloves and wipe a cuvette with a lab wipe to clean it and remove any fingerprints. A spectrometer is an analytical tool used across various scientific disciplines to measure how a substance interacts with light. Specifically, a UV-Visible Spectrometer measures the absorption or transmission of light in the ultraviolet (UV) and visible (Vis) regions of the electromagnetic. Meredith Juncker is a scientific researcher based in Brookline, Massachusetts. Clean and Prepare Containers and Control Samples 3. Here's a step-by-step guide on how to use a spectrophotometer.
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